Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Description

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

Specifications

• Color: Green
• Content And Storage: Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).
  
  Store as directed.
• Detection Method: Fluorescence
• Format: Tube
• Excitation/Emission: 490/525
• Flow Cytometer Laser Lines: 488
• For Use With (Application): Flow Cytometry
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Label or Dye: Alexa Fluor™ 488
• Product Type: TUNEL Assay Kit
• Product Line: APO-BrdU™
• Quantity: 60 Assays
• Species: Mouse
• Target: BrdU
• Label Type: Alexa Fluor Dyes

For Research Use Only. Not for use in diagnostic procedures.