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BioVendor, LLC Apoa Human Elisa BioVendor LLC
Supplier: BioVendor, LLC REH019R
The BioVendor ApoA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ApoA in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human ApoA coated on a 96-well plate. Standards and samples are pipetted into the wells and ApoA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman ApoA antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. One kit (96 wells). Biotin-labeled antibody. Calibration range: 0.082-20ng/ml. Limit of detection: 0.08ng/ml.
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