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Biotium Apoptosis & Necrosis Quantification Kit (50 assays)

Supplier:  Biotium 30017

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This kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy. The kit contains FITC-Annexin V which binds to apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface, and Ethidium Homodimer III (EthD-III) which is a membrane impermeant nucleic acid probe specific for dead cells. Staining is rapid, requiring only a single 15-30 minute staining step. FITC-Annexin V has an excitation emission of 498/517 nm, and EthD-III has an excitation/emission of 522/593 nm (and a large absorption peak at 279 nm) when bound to DNA. The kit has reagents sufficient for 50 assays.

Catalog No. 50-196-4473


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