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Biotium Apoptotic, Necrotic & Healthy Cells Quantitation Kit Plus (50 assays)
Supplier: Biotium 30066
This kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy. The kit contains Annexin V labeled with CF488A which binds to apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface, and Ethidium Homodimer III (EthD-III) which is a membrane impermeant nucleic acid probe specific for dead cells. Staining is rapid, requiring only a single 15-30 minute staining step. CF488A Annexin V has an excitation emission of 490/515 nm and is brighter and more photostable than FITC Annexin V. EthD-III has an excitation/emission of 522/593 nm (and a large absorption peak at 279 nm) when bound to DNA. The kit also contains a membrane permeable DNA dye Hocechst 3342 to stain all cell nuclei blue for analysis of healthy cells. The kit has reagents sufficient reagent for 50 assays.
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