Invitrogen™ Arg8-Vasopressin Competitive ELISA Kit

Catalog No.	Quantity
EIAAVP	96 Tests

Catalog No. EIAAVP Supplier Invitrogen™ Supplier No. EIAAVP

Description

ELISA

The multispecies Arg8-Vasopressin (AVP) Competivite ELISA with quantitates Arg8-Vasopressin in serum, plasma, and tissue culture media. Principle of the method: The Arg8-Vasopressin (AVP) Competitive ELISA research-use-only kit is designed to quantitatively measure AVP independent of species. An AVP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with goat anti-rabbit IgG antibodies. Rabbit polyclonal antibodies and AVP-peroxidase conjugate is added to the wells. The AVP conjugate and any AVP in the sample will compete to bind to the rabbit polyclonal AVP antibodies. After 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of AVP present in the sample. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Specifications

• Assay Range: 4.1-1000 pg/mL
• Assay Sensitivity: 3.67 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chromone
• Sample Type: Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ADH; antidiuretic hormone; arginine vasopressin; arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping); arginine vasopressin (neurophysin II, antidiuretic hormone, diabetes insipidus, neurohypophyseal); arginine vasopressin-neurophysin II precursor; Arginine-vasopressin; Arg-vasopressin; ARVP; Avp; AVP-neurophysin II precursor; AVP-NPII; AVRP; Copeptin; DI; neurohypophyseal; Neurophysin 2; Neurophysin-I; Neurophysin-II; prepro-arginine-vasopressin-neurophysin II; prepro-AVP-NP II; prepropressophysin; preprovasopressin-neurophysin; Vas; Vasopressin; vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin II-copeptin; vasopressin-neurophysin prepropeptide; VP; VspADH; antidiuretic hormone; arginine vasopressin; arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping); arginine vasopressin (neurophysin II, antidiuretic hormone, diabetes insipidus, neurohypophyseal); arginine vasopressin-neurophysin II precursor; Arginine-vasopressin; Arg-vasopressin; ARVP; Avp; AVP-neurophysin II precursor; AVP-NPII; AVRP; Copeptin; DI; neurohypophyseal; Neurophysin 2; Neurophysin-I; Neurophysin-II; prepro-arginine-vasopressin-neurophysin II; prepro-AVP-NP II; prepropressophysin; preprovasopressin-neurophysin; Vas; Vasopressin; vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin II-copeptin; vasopressin-neurophysin prepropeptide; VP; Vsp;
• Interassay CV: <9%
• Intraassay CV: <9%
• Kit Contents: Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Extraction Solution, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: 2-8°C
• Target: AVP
• Test Time: 1 hr 20 min
• Total Assay Time: 16 hr