Invitrogen™ Human Arginase-1 ELISA Kit

Catalog No.	Quantity
BMS2216	96 Tests

Catalog No. BMS2216 Supplier Invitrogen™ Supplier No. BMS2216

Description

ELISA

The Arginase-1 Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Arginase-1.

Principle of the method

The Human Arginase-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Specifications

• Accession Number: P05089
• Assay Range: 78.1-5000 pg/mL
• Assay Sensitivity: 20.81 pg/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Metabolic enzyme
• Sample Type: Cell Lysates, Plasma, Serum
• Substrate Type: Adaptor Proteins
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AI; A-I; AI type I arginase; AI256583; Arg1; Arg-1; Arginase; arginase 1; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; arginase-1; HGNC:663; Liver Arginase; Liver-type arginase; PGIF; similar to arginase, type I; Type 1 Arginase; type I arginaseAI; A-I; AI type I arginase; AI256583; Arg1; Arg-1; Arginase; arginase 1; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; arginase-1; HGNC:663; Liver Arginase; Liver-type arginase; PGIF; similar to arginase, type I; Type 1 Arginase; type I arginase;
• Gene ID (Entrez): 383
• Gene Symbol: ARG1
• Interassay CV: 8.5%
• Intraassay CV: 6.6%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: arginase, liver; liver-type arginase; type I arginase
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: Arginase 1
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min