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Montana Molecular Borealis Arrestin V2R Assay - 10mL

Supplier: Montana Molecular D2000G010

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Vasopressin Receptor (V2R) assay. Add the unmodified V2R receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, V2R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
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