Invitrogen™ Asymmetric Di-Methyl Arginine Competitive ELISA Kit

Catalog No.	Quantity
EEL009	96 Tests

Catalog No. EEL009 Supplier Invitrogen™ Supplier No. EEL009

Description

ELISA

The multispecies Asymmetrical Dimethylarginine (ADMA) ELISA quantitates ADMA in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Asymmetrical Dimethylarginine (ADMA) independent of species. An ADMA standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to ADMA is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of ADMA in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Asymmetrical dimethylarginine (ADMA) is an L-arginine analogue twice methylated in an asymetric pattern. ADMA can be found naturally in the body and can contribute to endothelial and cardiovascular disease.

Specifications

• Assay Range: 15.63-1000 ng/mL
• Assay Sensitivity: 9.38 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ADMA; ArginineADMA; Arginine;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Asymmetric Di-Methyl Arginine
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min