Sigma Aldrich Fine Chemicals Biosciences ATP Bioluminescence Kit Su

Supplier:  Sigma Aldrich Fine Chemicals Biosciences CS00121KT

Adenosine triphosphate (ATP) is a key molecule that stores energy within cells and is considered as the universal energy currency for all living organisms. ATP has been used as a marker of viability as living cells have high ATP content whereas dead cells lose their ability to produce ATP. ATP levels are therefore proportional to the amount of living cells in a sample. The ATP Bioluminescence Kit provides a simple and quick assay to quantify ATP and also to measure cell viability based on ATP content. This assay is less prone to artifacts than other viability assay methods. Intracellular ATP is released by permeabilization SOLUTIONon and measured using a bioluminescence assay owing to the ability of luciferase to produce light in the presence of its substrate D-luciferin and ATP. The kit has the following linear detection range0 - 60 000 cells (tested on TF-1 cells)1nM-10muM ATP

Catalog No. 50-290-8213