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Sigma Aldrich Fine Chemicals Biosciences ATP Cell Viability Lucifer
Supplier: Sigma Aldrich Fine Chemicals Biosciences SCT149
Because ATP is an indicator of metabolically active cells the number of viable cells can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of firefly luciferases use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. No cell washing medium removal and multiple pipetting are required. The kit can be used to detect as little as a single cell or 0.01 picomoles of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells the assay has wide applications ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.The ATP C
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