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New England Biolabs, Inc. Authenticase - 25 reactions
Supplier: New England Biolabs, Inc. M0689S
Authenticase is a mixture of enzymes capable of recognizing mismatch and indel regions on a double-stranded DNA and creating double-stranded cleavages around these regions and with a limited non-specific activity on homoduplex regions of DNA. The regions of mismatch or indel could range from at least 1 to 10 bps. The activity of cleavage on mismatch/Indel regions can be utilized in the process of oligo-based PCR gene synthesis to improve the accuracy of PCR amplicons. Alternatively, Authenticase treated products can be analyzed to determine the popularity of heteroduplex of a PCR pool amplified from enzyme-based edited genome.
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