Applied Biosystems™ AutoLys M TubeLifter

Catalog No.	Quantity
A37956	1 each

Catalog No. A37956 Supplier Applied Biosystems™ Supplier No. A37956

Description

The AutoLys M TubeLifter enables a rack of 24 AutoLys M Tubes to be simultaneously 'lifted' and readied for centrifugation.

Once centrifugation is complete the rack of tubes is returned to the TubeLifter and the inner tube of each AutoLys M Tube is separated from the outer tube. The outer tube contains the lysate that is processed further using the MagMAX FFPE DNA/RNA Ultra Kit to recover the nucleic acids.

Specifications

• For Use With (Equipment): AutoLys TubeLifter System
• Quantity: 1 each
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

Can I use a heat block for the AutoLys M tube incubation?

No. The AutoLys M tubes don't fit in a standard 1.5 mL or 2.0 mL heat block. We recommend incubating the tubes in the tube racks in an incubator or oven.

What kind of centrifuge do I need to spin down the AutoLys M Tubes (Cat. No. A38738)?

Any centrifuge with plate adapters will work. The AutoLys M Tube Racks fit nicely into those adapters.

Do I need to buy the AutoLys M TubeLifter (Cat. No. A37956) or AutoLys M Tube Pliers (Cat. No. A38261) to use the AutoLys M Tubes (Cat. No. A38738)?

No, the AutoLys M Tubes can be lifted manually, but the tools significantly reduce hand strain and increase ease of use as well as throughput.

Do the AutoLys M Tubes come with the MagMAX FFPE DNA/RNA Ultra Kit?

No, they have to be purchased separately together with some other products that are necessary for using the AutoLys M Tube system efficiently:
- AutoLys M Tubes and Caps (Cat. No. A38738)
- Autolys M Tube Locking Lid (Cat. No. A37954)
- AutoLys M TubeLifter (Cat. No. A37956)
- AutoLys M Tube Pliers (Cat. No. A38261)
- AutoLys M Tube Racks (Cat. No. A37955)

How does using the the AutoLys M Tube system help in increase the DNA/RNA yield from FFPE samples?

The FFPE-associated wax and debris are held in the upper chamber of the AutoLys M tube while the lysate passes through. Nucleic acids can then be purified from the clarified lysate. The AutoLys M tubes thus reduce manipulation of the sample eliminating the need to pellet the tissue and wash, so tissue loss is minimized

Can I use the AutoLys M Tube system with any of your FFPE sample prep kits or has it only been optimized for the MagMAX FFPE DNA/RNA Ultra Kit?

The AutoLys M Tube system has only been tested with the MagMAX FFPE DNA/RNA Ultra Kit, but it may also work with our RecoverAll Total Nucleic Acid Isolation Kit for FFPE .

I am planning on using your MagMAX FFPE DNA/RNA Ultra Kit. I don't want to perform a deparaffinization step using organic solvents, is there an alternative reagent or method I can use?

Yes, we offer the AutoLys M Tube system, which creates cleared lysates from FFPE tissue samples without the need for deparaffinization or wash steps, while increasing DNA and RNA yields.

For Research Use Only. Not for use in diagnostic procedures.