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Description
Thermo Scientific™ Pierce Avidin and Conjugates include purified avidin protein from hen egg whites, plus avidin-HRP, avidin-AP and other conjugated forms for use in biotin-based detection methods.
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
Highlights:
- Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10
- Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin
- Affordable – significantly less expensive than recombinant streptavidin
- ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
- Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays
Specifications
Specifications
| Quantity | 2 mg |
| Target Molecule | Biotin |
| Content And Storage | Store at -20°C. Shipped at ambient temperature. |
| Specificity | Biotin |
| Enzyme | HRP (Horseradish Peroxidase) |
| Form | Solid |
| For Use With (Application) | Western Blot, ELISA, Immunohistochemistry |
| Substrate | Avidin |
| Label or Dye | HRP (Horseradish Peroxidase) |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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