Axygen™ AxyPrep Mag™ Tissue Genomic DNA Kits

Description

For isolation of genomic DNA from Rodent tail tissues

The AxyPrep MAG Tissue-Blood gDNA Kit utilizes a magnetic beads-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instruction to extract gDNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL tubes. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. Genomic DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the gDNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.

• Expanded protocol for animal tissue, cultured cells, buccal swabs, and blood

Specifications

• Format: 96-well Plate, Tube
• For Use With (Application): Isolation of genomic DNA from Rodent tail tissues
• Includes: 125 mL TB1 Buffer, 120 mL TB2 Buffer, 88 mL Wash Buffer, 10 mL Proteinase K solution, 4.4 mL FB particles, 120 mL Elution Buffer, 1 protocol
• No. of Reactions: 384 Preps
• Product Type: Kit