Sigma Aldrich Azide-A-Dsbso Crosslinker

Catalog No.	Quantity
90-962-9100MG	50 mg

Catalog No. 90-962-9100MG Supplier Sigma Aldrich Supplier No. 909629100MG

Description

MilliporeSigma Sigma Organics products encompass a wide range of quality reagents, solvents, catalysts, and building blocks for organic synthesis. From benchtop discovery to process development and scale-up, Sigma Organics solutions are built to meet the needs of synthetic chemists.

Azide-A-DSBSO (azide-tagged, acid-cleavable disuccinimidyl bissulfoxide) Crosslinker is a homobifunctional, membrane-permeable, enrichable disulfoxide-containing crosslinker for analysis of protein-protein interactions (PPIs) through crosslinking mass spectrometry (XL-MS). Azide-A-DSBSO possesses two N-hydroxysuccinimide (NHS) ester groups for targeting amines, a ≥14 +a spacer length, two symmetrical acid-cleavable C-S bonds, and a central bioorthogonal azide tag. After reacting with lysine (Lys) residues, the azide tag permits selective enrichment of crosslinked proteins or peptides with a biotin-conjugated alkyne probe to enhance their detection. Additionally, the post-cleavage spacer yields tagged peptides for unambiguous identification by collision-induced dissociation in tandem MS. Azide-A-DSBSO Crosslinker has mapped in vivo PPIs for target protein complexes and whole proteomes fro mLiving cells. It will also be a useful proteomics tool in the quest for targeting undruggable protein targets.Technology Spotlight: Cross-Linkers for Elucidation of Protein-Protein InteractionsA New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein-Protein Interactions in Living CellsSynthesis of two new enrichable and MS-cleavable cross-linkers to define protein-protein interactions by mass spectrometrySubject to US Patent Application #15/275,001 of the Regents of the University of California

Specifications

• CAS: 2133345-76-1
• Molecular Formula: C24H29N3O9
• Linear Formula: C27H18B2ClF8N5Pd
• Quantity: 50 mg
• Synonym: 7-(2-(2-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)-2-oxoethoxy)ethoxy)heptanoic acid; Crosslinker-E3 ligase ligand conjugate; Pomalidomide conjugate; Pomalidomide-2-2-6-acid; Protein degrader building block for PROTAC(R) research; Template for synthesis of targeted protein degrader
• Molecular Weight (g/mol): 503.5 g/mol