Gibco™ B-27™ Supplement (50X), serum free

Catalog No.	Quantity
17-504-044	10 mL
17-504-001	100 mL

Catalog No. 17-504-044 Supplier Gibco™ Supplier No. 17504044

Description

The original Gibco B-27 Supplement, optimized for serum-free culture of neurons, supports the growth and viability of neurons and many different cell types.

This original and genuine B-27 Supplement is a cGMP-manufactured, serum-free solution designed to ensure the consistent, reliable, and high-quality growth of embryonic, post-natal, and adult hippocampal neurons, as well as other CNS neurons. With a proven track record, B-27 Supplement supports both low- and high-density cultures, ensuring viability in serum-free media. Each batch undergoes rigorous performance testing to confirm its ability to successfully culture hippocampal neurons, providing you with the confidence and dependability you need for your research.

• Dependable consistency—every lot is performance-tested for reliable results
• High quality—cGMP manufacturing provides the highest standards of quality
• Versatile usage—ideal for use with serum-free media such as Neurobasal Medium or Neurobasal-A Medium, without the need for an astrocyte feeder layer. Supports the growth and viability of neurons, as well as many other cell types differentiated from PSCs.
• Proven performance—a proprietary formulation; the first and most cited neuronal cell culture supplement, trusted by researchers worldwide

Choose B-27 Supplement for the authentic solution that sets the standard for neuronal cell culture.

Order Info

Dry Ice

Specifications

• Content And Storage: Store in freezer (-5 to -20°C) and protect from light.
• Cell Type: Neural Cells
• Classification: Serum-free
• Concentration: 50 X
• Culture Type: Mammalian Cell Culture
• Form: Liquid
• Product Type: Cell Culture Supplement
• Serum Level: Serum-Free
• Sterility: Sterile
• pH: 6 to 8
• Product Line: B-27
• Quantity: 10 mL
• Shipping Condition: Dry Ice
• Source: Serum-Free
• Tested For: Endotoxin, Performance, Sterility, Mycoplasma

Frequently Asked Questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

How long does media supplemented with B-27 Supplement last after supplementation?

Once B-27 Supplement has been added to a basal medium, the medium should be used within 1 to 2 weeks. If it will take longer than that to use up the medium, we recommend that you aliquot the B-27 Supplement and supplement smaller volumes of medium as needed.

What is the difference between B-27 Plus Supplement and classic B-27 Supplement?

B-27 Plus Supplement is an optimized formulation of classic B-27 Supplement with a streamlined manufacturing process. No small molecules or growth factors have been added.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.

In which protocols would you recommend substituting B-27 Plus Supplement and Neurobasal Plus Medium for classic B-27 and neurobasal products?

We recommend using B-27 Plus Supplement and Neurobasal Plus Medium instead of the regular versions of B-27 Supplement and Neurobasal Medium products (not specialty versions like minus Insulin, minus Vitamin-A or Neurobasal-A) in protocols used for:

- Maintenance of primary rat, mouse and human PSC-derived and fetal-derived neurons
- Differentiation of human PSC-derived and fetal-derived neural stem cells (NSCs) to neurons

Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.

What B-27 supplements do you offer?

We offer the following B-27 supplement products, which are developed for specific applications:

- Gibco B-27 Serum-free Supplement (Cat. No. 17504044): Complete formulation for the growth of long-term viability of neurons.
- Gibco B-27 Supplement minus AO (antioxidants) (Cat. No. 10889038): Five antioxidants are removed. The supplement is ideal in studies of oxidative damage, apoptosis, or applications in which free radical damage to neurons occurs.
- Gibco B-27 Supplement minus Insulin (Cat. No. A1895601): Insulin is removed. The supplement is ideal for studies of insulin secretion and insulin receptors.
- Gibco B-27 Supplement minus Vitamin A (Cat. No. 12587010): Vitamin A which induces neural differentiation is removed. The supplement is ideal for proliferation of stem cells.

Which supplement can I add to Neurobasal Medium to culture my neural cells?

As a general guideline, we recommend using Gibco B-27 Plus Supplement to culture neural stem cells, hippocampal, and other CNS neurons.

- For neuroblastomas, or post-mitotic neurons from both PNS and CNS, Gibco N-2 Supplement can be used.
- For primary glial cells (astrocytes) or tumor cell lines of astrocytic phenotype (astrocytes and gliomas), or oligodendrocytes, Gibco G-5 Supplement can be used.

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.