This ELISA kit is designed for the quantitative detection of human B-hexosaminidase A (B-Hex A) in serum and plasma samples using a sandwich assay format With a detection range of 1 56 mIU/mL to 100 mIU/mL and a sensitivity of 0 39 mIU/mL the kit provides reliable measurements for low-abundance targets The procedure requires 50-100 L of sample volume and features an incubation time ranging from 1 to 5 hours optimized for efficient workflow integration Detection is performed at 450 nm wavelength ensuring precise optical measurement This kit detects BHex A with high specificity and reproducibility making it suitable for research applications involving lysosomal enzyme analysis including metabolic disorder studies and diagnostic screening