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Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit
Description
Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents.
Highlights:
Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations
Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris
Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels
Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes
Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications
Convenient, ready-to-use formats:
- B-PER and B-PER II (2X) Reagents are free of enzymatic components
- B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking
- B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery
Includes:
Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Specifications
Specifications
Quantity | 250 mL |
Format | Liquid |
Product Type | Bacterial Protein Extraction Kit |
Volume (Metric) | 250 mL |
Content And Storage | Sufficient For: 60 g cell paste • B-PER Reagent, 250 mL • Lysozyme, 50 mg/mL, 0.5 mL • DNase I, 2500 U/mL, 0.5 mL Store B-PER Reagent at ambient temperature. Store Lysozyme and DNaseI at -20°C. |
Product Line | B-PER™ |
For Research Use Only. Not for use in diagnostic procedures.
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