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IBL America Bordetella Pertussis Toxin IgG ELISA
Supplier: IBL America IB05014
Enzyme immunoassay (ELISA) for the determination of human IgG antibodies in serum or plasma directed against Bordetella pertussis toxin. For research use only, not for use in diagnostic procedures.Thereaction is based on the specific interaction of antibodies with their corresponding antigen.The test strips of the microtiter plate are coated with specific antigens of the pathogen ofinterest. If antibodies in the sample are present, they bind to the fixed antigen. Asecondary antibody, which has been conjugated with the enzyme alkaline phosphatase,detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. Thesignal intensity of this reaction product is proportional to the concentration of the analyte inthe sample and is measured photometrically.
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