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ACROBiosystems ACROBiosystems Biotinylated Human B7-2 / CD86 Protein, Fc,Avitag™

Supplier:  ACROBiosystems CD6H82F525UG

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Recombinant Protein;25UG;Biotinylated Human B7-2, Fc,Avitag (CD6-H82F5) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Pro 247 (Accession # AAH40261).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 53.8 kDa. As a result of glycosylation, the protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc,Avitag (Cat. No. CD6-H82F5) with a linear range of 19-156 ng/mL (QC tested).;Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-2, Fc,Avitag (Cat. No. CD6-H82F5) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 μg/mL (Routinely tested).

Catalog No. 50-195-7421


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