Bac-Off (4mL or 10mL) is a widely used antibiotic ideal for use in your cell culture experiments -- inhibiting the growth of bacterial agents and controlling contamination without harming the cells. By nature, cells can enclose bacteria adherent to their luminal surface and sink them into the cytoplasm. Cells containing bac-vacuoles at high multiplicities are adversely affected. Bac-Off is formulated at MIC - minimum inhibitory concentration. Its bacteriostatic (working strength 1:500) and bactericidal (double strength 1:250) activity is achieved by inhibiting the bacterial enzymes topoisomerase (II & IV). Unlike most antibiotics, it won't harm mammalian cells at typical working strengths. Bac-Off may also be used at 10 times working strength (1:50 v/v) at which it exhibits mycoplasmacidal properties. Bac-Off contains the synthetic fluoroquinone Ciprofloxacin, with a wide range of in vitro activity against most Gram-negative and Gram-positive microorganisms.