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Invitrogen™ Bac-to-Bac™ C-His TOPO™ Expression System Non-distribution product as customer accommodation.

Bac-to-Bac™ C-His TOPO™ Expression System

Manufacturer:  Invitrogen™ A11100

Catalog No. A11100


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Description

Description

Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. New: pFastBacTOPO™ vectors!


Reliable and fast protein expression
The Bac-to-Bac™ Baculovirus Expression System is faster and easier than traditional baculovirus expression, and it maintains high levels of protein expression. Bac-to-Bac™ relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells. The Bac-to-Bac™ Baculvirus Expression System is highly regarded in academic Literature. Approximately 100 citations every year since 2000!

Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. This requires a time-consuming plaque assay. Bac-to-Bac™'s pFastBac™ vector, however, recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid. Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. You'll save weeks of precious time. Collect a pure P2 baculovirus stock on Day 10 without the necessity of tedious plaques assay (See Figure 1)

High expression, easy screening
pFastBac™ uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Sf21, and High Five™ cells.

New additions:
Bac-to-Bac™'s pFastBac vectors are now available as pFastBacTOPO™ vectors too. pFastBacTOPO™ vectors are Blunt™ TOPO™ vectors and are supplied with Mach1™-T1R E. coli for easy cloning and pFastBac⁄GOI propagation. The new vectors enable you to:

  • Amplify your gene of interest (GOI) with a PCR enzyme of highest fidelity such as Accuprime™ Pfx SuperMix.

  • TOPO™ clone the blunt-end PCR product into the new vector in only 5 minutes!

  • Visualize colonies 8 hours after plating on ampicillin selective plates because Mach1™-T1R E. coli cells have a faster doubling time compared to other standard cloning strains.

  • With the Bac-to-Bac™ C-His TOPO™ cloning or expression kit, you can produce a C-terminal His-fusion protein with a TEV cleavage site to purify with nickel-chelating resins (Invitrogen’s ProBond™ Purification System) and generate a native protein with the aid of Invitrogen’s popular AcTEV™ Protease. This is the Invitrogen’s only vector that contains a C-terminal TEV cleavage site!

    The new vectors combine the TOPO™ cloning technology with the highly regarded Bac-to-Bac™ baculovirus expression features to enable easy cloning and high-level protein expression.
    The pFastBacTOPO™ vectors are offered as cloning and expression kits.
  • Specifications

    Specifications

    Bac-to-Bac™ C-His TOPO™ Expression System, 20 rxns, Polyhedrin Promoter, Cell-Based Expression Mechanism, Ampicillin (AmpR), Gentamicin (GmR) Antibiotic Resistance, His Tag (6x) Protein Tag, Used For Protein Expression, Viral Vaccine Production
    20 rxn(s)
    Vector kit for 20 topo cloning reactions, competent cells, MAX Efficiency™ DH10Bac™ Competent E, 1mL Cellfectin™ II Reagent
    Bac-to-Bac™ C-His TOPO™ Expression System
    Protein expression
    20 rxn(s)
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