Invitrogen Bac-to-Bac N-His TOPO Cloning Kit
Bac-to-Bac N-His TOPO Cloning Kit
Manufacturer: Invitrogen A11099
DescriptionBac-to-Bac Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. New: pFastBacTOPO vectors!
Reliable and fast protein expression
The Bac-to-Bac Baculovirus Expression System is faster and easier than traditional baculovirus expression, and it maintains high levels of protein expression. It is based on site-specific transposition in E. coli rather than homologous recombination in insect cells. The Bac-to-Bac Baculvirus Expression System is highly regarded in academic Literature. At least 80 citations every year since 2000!
Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. This requires a time-consuming plaque assay. Bac-to-Bac's pFastBac vector, however, recombines with the parent bacmid in DH10Bac E. coli competent cells to form an expression bacmid. Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. You'll save weeks of precious time. Collect a pure P2 baculovirus stock on Day 10 without the necessity of tedious plaques assay (See Figure 1)
High expression, easy screening
pFastBac uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Sf21, and High Five cells.
Bac-to-Bac's pFastBac vectors are now available as pFastBacTOPO vectors too. pFastBacTOPO vectors are Blunt TOPO vectors and are supplied with Mach1-T1R E. coli for easy cloning and pFastBac⁄GOI propagation. The new vectors enable you to:
The new vectors combine the TOPO cloning technology with the highly regarded Bac-to-Bac baculovirus expression features to enable easy cloning and high-level protein expression.
The pFastBacTOPO vectors are offered as cloning and expression kits.
|Ampicillin (AmpR), gentamicin (GmR)|
|Protein expression, viral vaccine production, protein biology, protein expression, insect expression, high-level protein production in insect cells, baculovirus production and titering, bioproduction, viral vaccine production|
|Vector kit for 20 topo cloning reactions: pFastBac/NT-TOPO™ Vector containing the N-terminal His-Tag and TEV cleavage site, pFastBac/NT-Gus control plasmid (Control expression vector), 10x PCR, dNTP Mix, salt solution, sterile water, control PCR template, polyhedrin forward primer, SV40 pA reverse primer. Kit containing competent cells (20 reactions): One Shot™ Mach1-T1R chemically competent E. coli vector kit: store at –20°C|
|Polyhedrin forward, SV40 pA reverse|
|–30 to –10°C|
|Bac-to-Bac™ N-His TOPO™ Cloning Kit, 1 Kit, 20 rxns, High Five™, Sf21, Sf9 Cell Line, Baculovirus Cell-Based Expression, Polyhedrin Promoter, His Tag Protein, Blunt TOPO™ Cloning Method, pFastBac/NT-TOPO Vector, Six Months Shelf Life, –30 to –10°C Storage Temperature, Used for Protein Expression and Viral Vaccine Production|
|Bac-to-Bac™ N-His TOPO™ Cloning Kit|
Safety and Handling
ShelfLife : 6 Month(s)
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For Research Use Only. Not for use in diagnostic procedures.