In addition to various cell type-specific kits for primary endothelial cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian endothelial cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for endothelial cells. Benefits:Optimization can be performed within 1 experiment Detailed protocol guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90%Viabilities up to 85%Applications:Kit suited for endothelial cells from different mammalian species and various organsAlready tested for human pulmonary artery endothelial cells