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Apexbio Technology LLC BCA Protein Assay Kit 500T
Supplier: Apexbio Technology LLC K4101200
BCA fully named Bicinchoninic Acid Assay is a protein quantification method based on the biuret reaction principle Under alkaline conditions protein peptide bonds reduce Cu2 (from copper sulfate) to Cu Subsequently BCA chelates Cu to form a purple-colored complex which strongly absorbs light at a wavelength of 562 nm The intensity of the colored complex is proportional to protein concentration as the reduction of Cu2 or the amount of Cu formed directly reflects the amount of protein present in the solution Thus protein concentration in an unknown sample can be determined by measuring absorbance This assay kit features easy operation high stability high sensitivity and excellent compatibility It can detect protein concentrations as low as 25 g/mL with a minimum detection limit as low as 0 5 g protein It requires a sample volume of only 1 20 L The assay shows good linearity in the concentration range of 50 2000 g/mL
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