Invitrogen™ Human BCAM ELISA Kit

Catalog No.	Quantity
EHBCAMX10	10 x 96 Tests

Catalog No. EHBCAMX10 Supplier Invitrogen™ Supplier No. EHBCAMX10

Description

ELISA

The Human Basal Cell Adhesion Molecule (Hu BCAM) ELISA quantitates Hu BCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BCAM. Principle of the method The Human BCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P50895
• Assay Range: 40.96-10000 pg/mL
• Assay Sensitivity: 40 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cell Structure/Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1200005K12Rik; antigen identified by monoclonal antibody F8; AU; Auberger B antigen; basal cell adhesion molecule; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule Lutheran blood group; BCAM; B-CAM; B-CAM cell surface glycoprotein; B-cell adhesion molecule; CD239; F8/G253 antigen; glycoprotein 95kDa; Gplu; LU; lutheran antigen; Lutheran blood group; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; Lutheran blood group variant LUGA; lutheran glycoprotein; MSK191200005K12Rik; antigen identified by monoclonal antibody F8; AU; Auberger B antigen; basal cell adhesion molecule; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule Lutheran blood group; BCAM; B-CAM; B-CAM cell surface glycoprotein; B-cell adhesion molecule; CD239; F8/G253 antigen; glycoprotein 95kDa; Gplu; LU; lutheran antigen; Lutheran blood group; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; Lutheran blood group variant LUGA; lutheran glycoprotein; MSK19; AU; CD239; LU; MSK19
• Gene ID (Entrez): 4059
• Gene Symbol: BCAM
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: BCAM
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min