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Bioworld BCIP, 500 mg
Supplier: Bioworld 215300502
BCIP Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. Technical Information:Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeSolubility (0.10% DMF): Pass (Clear/Haze-free)Melting Point: 195-197CA260 (1mg/ml, DMF): 0.010Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
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