Abnova™ BCL6 Split CISH Probe

Catalog No.	For Use With (Application)
89-335-534	CISH-Ce

Catalog No. 89-335-534 Supplier Abnova™ Supplier No. CS0003

Description

BCL6 Split CISH Probe is designed for the qualitative detection of human BCL6 gene at 3q27.3 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the BCL6 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the BCL6 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving BCL6 gene region, two red/green fusion signals appear.
Aberrant situation: One BCL6 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-Ce
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.