BD Ac-DEVD-AMC Caspase-3 Substrate

Catalog No. BDB556449

Supplier: BD 556449

Description

Synthetic tetrapeptide fluorogenic substrate for Caspase-3 (CPP32) containing the amino acid sequence of the PARP cleavage site at Asp-216

Members of the ICE/CED-3 cysteine protease family have key roles in inflammation and mammalian apoptosis. The ICE family member Caspase-3 (also known as CPP32, Yama, apopain) is activated early in apoptosis and appears to be involved in the proteolysis of several important molecules, including poly (ADP ribose) polymerase (PARP). Activated Caspase-3 cleaves PARP from its 116 kDa to an 85 kDa residual fragment. The cleavage site in PARP is C-terminal to Asp-216. The upstream sequence of the cleavage site, DEVD (Asp-Glu-Val-Asp), is utilized as a basis for the highly specific Caspase-3 substrate, Ac (N-acetyl)-DEVD-AMC (7-amino-4-methylcoumarin).

• Format: Purified
• Storage buffer: Lyophilized powder

Functional assay

Specifications

• Form: Lyophilized
• For Use With (Application): For Caspase-3, fluorescence quantitation, used in protease assays
• Quantity: 1 mg
• Substrate Type: Caspase Substrate
• Includes: Amino acid sequence of the PARP cleavage site at Asp 216
• Molecular Weight (g/mol): 694
• Storage Requirements: -20°C
• Substrate Quantity: 1mg

Research use only.