BD Fixable Viability Stain 570
For discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. Reacts with and covalently binds to cell-surface and intracellular amines.
Manufacturer: BD 564995
- Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells
- Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells
- Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence
- Excited by Yellow-Green laser (with an excitation maximum of 547nm), but is also well-excited by the Blue laser
- Fluorescence emission maximum of 573nm
- Applications: Flow cytometry, Intracellular staining
|Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)|
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