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BD BD Biosciences Fixable Viability Stain 620

For discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications

Manufacturer:  BD 564996

Catalog No. BDB564996

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  • Reacts with and covalently binds to cell-surface and intracellular amines
  • Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells
  • Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells
  • Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence
  • Excited by Yellow-Green laser (with an excitation maximum of 523nm), but is also well-excited by the Blue laser
  • Fluorescence emission maximum of 617nm
  • Applications: Flow cytometry, Intracellular staining


Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
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