BD BD Biosciences Fixable Viability Stain 620

Description

For discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications

• Reacts with and covalently binds to cell-surface and intracellular amines
• Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells
• Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells
• Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence
• Excited by Yellow-Green laser (with an excitation maximum of 523nm), but is also well-excited by the Blue laser
• Fluorescence emission maximum of 617nm
• Applications: Flow cytometry, Intracellular staining

Specifications

• Quantity: 100 μg
• For Use With (Application): Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)

RUO