Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Staining Reagents
  5. Stains and Dyes
  6. BDB562247

BD Fixable Viability Stain 450

Catalog No. BDB562247 Shop All BD Biosciences Products
Click to view available options
Quantity:
0.01 g

Description

Useful to discriminate viable from non-viable mammalian cells in multicolor flow cytometric application

Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while maintaining stable FVS450 fluorescence.

  • Excited by the violet laser (with an excitation maximum of 406nm) and has a fluorescence emission maximum of 450nm
  • Contains a dye that reacts with and covalently binds to cell surface and intracellular amines

TRUSTED_SUSTAINABILITY

Specifications

Type Fixable Viability Stain
Quantity 0.01 g
For Use With (Application) Research
Storage Requirements -80°C
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.