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BD Horizon™ Fixable Viability Stain 520

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Fixable viability Stain 520
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BDB564407 Fixable viability Stain 520
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Description

Useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications

  • Reacts with and covalently binds to cell-surface and intracellular amines
  • Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells
  • Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells
  • Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence
  • Excited by Blue laser (with excitation maximum of 498nm) and has fluorescence emission maximum of 521nm

Flow cytometry, Intracellular staining

TRUSTED_SUSTAINABILITY

Specifications

Type Fixable Viability Stain

RUO

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