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BD Human C4a ELISA Kit

Catalog No. BDB550947
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Quantity:
1 Plate
Toxic, avoid contact with skin, eyes, or clothing
Toxic, avoid contact with skin, eyes, or clothing

Description

Requires

Microplate reader capable of measuring absorbance at 450nm, precision pipettes to deliver 50μL and 100μL volume, adjustable 1mL, 5mL, 10mL, 25mL pipettes for reagent preparation, deionized or distilled water, wash bottle or automated microplate washer, log-log graph paper or automated data reduction, tubes to prepare standard dilutions, laboratory timer, absorbent paper

Quantitative determination of human C4a and C4a-desArg in human EDTA plasma, serum and other biological samples

Utilizes monoclonal antibody specific for human C4a/C4a-desArg coated on 96-well plate. Standards and samples are added to wells, and any C4a-desArg present binds to immobilized antibody. The wells are washed and a mixture of biotinylated polyclonal anti-human C4a antibody and streptavidin-horseradish peroxidase is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and substrate solution is added, which produces a blue color in direct proportion to the amount of C4a-desArg present in initial sample. Stop Solution changes the color from blue to yellow, wells are read at 450nm

  • Solid phase sandwich ELISA
  • Signals activation of classical or lectin complement pathways in several autoimmune diseases including rheumatoid arthritis, lupus erythematosus, and acute glomerulonephritis as well as in early stages of organ transplant rejections
  • Weak multifunctional proinflammatory mediator by increasing vascular permeability, and to be spasmogenic

TRUSTED_SUSTAINABILITY

Specifications

Antigen C4a
Cross Reactivity Human
Includes Wash concentrate (20X), stop solution, Enzyme concentrate Streptavidin-HRP, ELISA diluent, standard/Sample diluent, TMB one-step Substrate reagent, Human C4a Antibody coated plate, detection Antibody Biotin Anti-Human C4a, Human C4a standard
Quantity 1 Plate
Sample Type Human EDTA Plasma, Serum, Biological Sample
Content And Storage 2°C to 8°C
For Use With (Application) Quantitative determination of Human C4a and C4a-desArg, ELISA
Product Type Human C4a ELISA kit

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Research use only

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