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BD Human IFN-γ ELISA Kit II

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Description

For in vitro quantitative determination of human interferons in serum, plasma, and cell culture samples

The OptEIA™ test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).

  • Reactivity: Human
  • Utilizes monoclonal antibody specific for IFN-γ coated on a 96-well plate

ELISA

Includes:

  • Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips x 8 wells) coated with anti-human IFN-Γ monoclonal antibody
  • Detection Antibody: 30mL of biotinylated anti-human IFN-Γ monoclonal antibody with ProClin™-150 as preservative
  • Standards: 4 vials lyophilized recombinant human IFN-Γ
  • Enzyme Concentrate (250x): 150μL of 250x concentrated Streptavidin-horseradish peroxidase conjugate with BSA and ProClin-300 as preservative
  • Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
  • ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
  • Wash Concentrate (20x): 100mL of 20x concentrated detergent solution with ProClin-150 as preservative
  • TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
  • Stop Solution: 13mL of 1 M phosphoric acid
  • Plate Sealers: 4 sheets with adhesive backing
TRUSTED_SUSTAINABILITY

Specifications

Antigen IFN
Cross Reactivity Human
Includes Human IFN-g Antibody coated plate, detection Antibody biotin Anti-Human IFN-g, Human IFN-gamma standard, TMB one-step Substrate reagent, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), ELISA Diluent, stop solution, standard/Sample Diluent
Quantity 2 Plates
Sample Type Serum, Plasma, Cell Culture
Content And Storage 2°C to 8°C
For Use With (Application) In-vitro quantitative determination of Human interferons, ELISA
Product Type Human IFN-gamma ELISA kit II

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Research Use Only

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