BD Human IL-6 ELISA Kit II

Description

Includes

2 plates of 96 breakable wells coated with anti-human IL-6 antibody; Detection antibody: Biotinylated anti-human IL-6; Enzyme Concentrate (250X): Horseradish peroxidase conjugate; Recombinant human IL-6, lyophilized, 4 vials; Standard Diluent; Wash Concentrate (20X); Substrate Reagent A; Substrate Reagent B; Stop Solution; Adhesive Plate Sealers

Requires

Microplate reader to read at 450nm, precision pipettes (50 and 100μL), adjustable 1mL, 5mL, 10mL, 25mL pipettes, deionized or distilled water, wash bottle or automated microplate washer, log-log graph paper or computer and software for ELISA data analysis, tubes to prepare standard dilutions, laboratory timer, absorbent paper

Quantitative determination of human IL-6 in serum, plasma, and cell culture supernatant

IL-6 specific monoclonal antibody binds to immobilized antibody coated on 96-wells plate. Wells are washed and streptavidinhorseradish peroxidase conjugate mixed with biotinylated anti-human IL-6 antibody is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450nm.

• Solid phase sandwich ELISA
• Standard Range: 4.7 - 300pg/mL
• Sensitivity: 4pg/mL

Specifications

• Antigen: IL-6
• Cross Reactivity: Human
• Includes: 2 Plates of 96 breakable well coated with Anti-Human IL-6 Antibody, Biotinylated Anti-Human IL-6, HRP Conjugate (250X), 4 vials recombinant Human IL-6 (lyophilized), standard Diluent, wash concentrate (20X), Substrate reagent A, Substrate reagent B, stop
• Quantity: 2 Plates
• Sample Type: Serum, Plasma, Cell Culture
• Content And Storage: 2°C to 8°C
• For Use With (Application): Quantitative determination of Human IL-6, ELISA
• Product Type: Human IL-6 ELISA kit II

Research use only