BD Human TNF ELISA Kit II

Description

For quantitative determination of human TNF in serum , plasma, and cell culture supernatant

The OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).

• Utilizes monoclonal antibody specific for TNF coated on a 96-well plate
• Developed for accuracy with serum and plasma specimens

ELISA

Includes:

• Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips × 8 wells) coated with anti-human TNF monoclonal antibody
• Detection Antibody: 30mL of biotinylated anti-human TNF monoclonal antibody containing FBS and ProClin™-150 as preservative
  Standards: 4 vials lyophilized recombinant human TNF
• Enzyme Concentrate: 150μL of 250× concentrated Streptavidinhorseradish peroxidase conjugate with BSA and ProClin-300 as preservative
• Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
• ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
• Wash Concentrate (20×): 100mL of 20× concentrated detergent solution
• TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
• Stop Solution: 13mL of 1 M phosphoric acid
• Plate Sealers: 4 sheets with adhesive backing

Specifications

• Antigen: TNF
• Cross Reactivity: Human
• Includes: Human TNF Antibody coated plate, detection Antibody Biotin Anti-Human TNF, Human TNF standard, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), TMB one-step Substrate reagent, stop solution, standard/Sample Diluent, ELISA Diluent
• Quantity: 2 Plates
• Sample Type: Serum, Plasma, Cell Culture Supernatant
• Content And Storage: 2°C to 8°C
• For Use With (Application): Quantitative determination of Human TNF, ELISA
• Product Type: Human TNF ELISA kit II

Research Use Only