Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. BDB550610

BD Human TNF ELISA Kit II

Catalog No. BDB550610 Shop All BD Biosciences Products
Change view
Click to view available options
Quantity:
2 Plates
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
BDB550610 2 Plates
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. BDB550610 Supplier BD Supplier No. 550610
Only null left
Add to Cart
Add to Cart

Description

For quantitative determination of human TNF in serum , plasma, and cell culture supernatant

The OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).

  • Utilizes monoclonal antibody specific for TNF coated on a 96-well plate
  • Developed for accuracy with serum and plasma specimens

ELISA

Includes:

  • Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips × 8 wells) coated with anti-human TNF monoclonal antibody
  • Detection Antibody: 30mL of biotinylated anti-human TNF monoclonal antibody containing FBS and ProClin™-150 as preservative
    Standards: 4 vials lyophilized recombinant human TNF
  • Enzyme Concentrate: 150μL of 250× concentrated Streptavidinhorseradish peroxidase conjugate with BSA and ProClin-300 as preservative
  • Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
  • ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
  • Wash Concentrate (20×): 100mL of 20× concentrated detergent solution
  • TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
  • Stop Solution: 13mL of 1 M phosphoric acid
  • Plate Sealers: 4 sheets with adhesive backing
TRUSTED_SUSTAINABILITY

Specifications

Antigen TNF
Cross Reactivity Human
Includes Human TNF Antibody coated plate, detection Antibody Biotin Anti-Human TNF, Human TNF standard, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), TMB one-step Substrate reagent, stop solution, standard/Sample Diluent, ELISA Diluent
Quantity 2 Plates
Sample Type Serum, Plasma, Cell Culture Supernatant
Content And Storage 2°C to 8°C
For Use With (Application) Quantitative determination of Human TNF, ELISA
Product Type Human TNF ELISA kit II

Research Use Only

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.