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BD Human TNF ELISA Kit II

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Description

For quantitative determination of human TNF in serum , plasma, and cell culture supernatant

The OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).

  • Utilizes monoclonal antibody specific for TNF coated on a 96-well plate
  • Developed for accuracy with serum and plasma specimens

ELISA

Includes:

  • Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips × 8 wells) coated with anti-human TNF monoclonal antibody
  • Detection Antibody: 30mL of biotinylated anti-human TNF monoclonal antibody containing FBS and ProClin™-150 as preservative
    Standards: 4 vials lyophilized recombinant human TNF
  • Enzyme Concentrate: 150μL of 250× concentrated Streptavidinhorseradish peroxidase conjugate with BSA and ProClin-300 as preservative
  • Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
  • ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
  • Wash Concentrate (20×): 100mL of 20× concentrated detergent solution
  • TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
  • Stop Solution: 13mL of 1 M phosphoric acid
  • Plate Sealers: 4 sheets with adhesive backing
TRUSTED_SUSTAINABILITY

Specifications

Antigen TNF
Cross Reactivity Human
Includes Human TNF Antibody coated plate, detection Antibody Biotin Anti-Human TNF, Human TNF standard, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), TMB one-step Substrate reagent, stop solution, standard/Sample Diluent, ELISA Diluent
Quantity 2 Plates
Sample Type Serum, Plasma, Cell Culture Supernatant
Content And Storage 2°C to 8°C
For Use With (Application) Quantitative determination of Human TNF, ELISA
Product Type Human TNF ELISA kit II

Research Use Only

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