Perm Buffer IV (10x), BD
For improved immunofluorescent staining and detection of certain intracellular phosphorylated proteins
Manufacturer: BD Biosciences 560746
BD Phosflow™ Perm Buffer IV (Perm Buffer IV) is intended for the permeabilization of paraformaldehyde-fixed single-cell suspensions prepared from lymphoid tissues, peripheral blood mononuclear cells (PBMC) and lysed whole blood cells.
- Treatment with buffer and subsequent washing with staining buffer enables access for fluorescent antibodies to stain intracellular molecules
- Buffer preserves light scattering properties of cells
- Providies capacity for intracellular immunofluorescent staining in preparation for Phosflow cytometric analysis
Flow Cytometry, Intracellular staining
|Used for the permeabilization of Paraformaldehyde-fixed single-cell suspensions prepared from lymphoid tissues, peripheral Blood mononuclear cells and lysed whole Blood cells, flow cytometry, intracellular staining|
|Cell permeabilization Buffer|
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Research Use Only