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BD Pharmingen™ Transcription-Factor Buffer Set
Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins
Supplier: BD 562574
Alert:
5x Perm/Wash Buffer contains ≤0.09% sodium azide. Sodium azide yields highly toxic hydrazoic azid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4x Fix/Perm Buffer contains diethylene glycol and formaldehyde and is harmful.
Includes: Fix/Perm Buffer (4X), Diluent Buffer, Perm/Wash Buffer (5X)
Description
This set is designed to minimize processing time, to reduce nonspecific staining, to increase resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. This is useful for flow cytometric
detection of many proteins. It supports multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage.
- High sensitivity for many transcription factors including FoxP3, RORgt, Bcl-6, and T-bet
- Compatible with many cell types, cell surface staining, and tandem dyes
- Size: 100 tests
Intracellular staining (flow cytometry)
Specifications
| Flow cytometry | |
| Fix/Perm Buffer (4X), Diluent Buffer, Perm/Wash™ Buffer (5X) | |
| 100 Tests |
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