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Invitrogen™ BDNF Rat ELISA Kit
GSA_VA

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ ERBDNF

Encompass

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Catalog No. ERBDNF


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Description

Description

The Rat Brain-Derived Neurotrophic Factor (Rt BDNF) ELISA quantitates Rt BDNF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt BDNF. Principle of the method The Rat BDNF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

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Specifications

Specifications

ELISA Kit
Colorimetric Microplate Reader
Biotin
Solid-phase sandwich ELISA
P23363
12pg/mL to 3,000pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-rat BDNF precoated 96-well strip plate, lyophilized recombinant rat BDNF standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrated buf
Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
1 hr. 20 min.
Rat
Rat
N/A
<10%
Rat
12 pg/mL
Colorimetric
96 Assays
This ELISA pair antibody detects rat and human BDNF. Other species not determined.
Growth Factors & Receptors
HRP
12.29-3000 pg/mL
BDNF ELISA Kit, 96 Assays, 1 Kit, Rat Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BDNF in Samples Using a Microplate Reader
24225
RUO
2°C to 8°C
4 hr. 45 min.
Rat and human BDNF
Neurobiology
<12%
96 Tests
Plasma, Serum, Supernatant
Bdnf
SDS
Documents
Product Certifications
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