Gibco™ BenchStable™ DMEM/F12

Catalog No.	Quantity
A4192001	500 mL
A4192002	10 x 500 mL

Catalog No. A4192001 Supplier Gibco™ Supplier No. A4192001

Description

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).

This DMEM/F-12 is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPEPS

Using BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

Specifications

• Cell Line: MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 9001: 2015 standard
• Product Line: BenchStable
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Green Features: ACT Ecolabel (Silver)
• Content And Storage: Store at room temperature. Protect from light.

Frequently Asked Questions (FAQs)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.

Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.

Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.

Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.

Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

How should I store BenchStable media? Can they be stored at 4 degrees C?

We recommend storing BenchStable media under ambient conditions (15-30 degrees C), protected from light. BenchStable media can alternatively be stored at 2-8 degrees C.

Can I store the box containing BenchStable medium in the refrigerator?

Yes, the box provided with our BenchStable media has properties that offer some resistance to moisture generated in a refrigerator environment.

My BenchStable medium arrived partially/completely frozen. Is it still stable?

Yes, BenchStable media should be stable for use if received partially or completely frozen.

Is degradation of BenchStable media due to light exposure only limited to fluorescent light?

Our in-house data, using refrigerated BenchStable media exposed to fluorescent light (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/benchstable-cell-culture-media.html#results), illustrates the necessity of having light protection. While we have not tested other light spectrums; we recommend storing BenchStable media protected from any light exposure.

How long can BenchStable media be exposed to light before cell growth is affected?

We have observed rapid degradation of several components within days from exposure of BenchStable media to common fluorescent light. We have also observed decreased cell growth using light-exposed media.

What is the recommended usage timeframe for BenchStable media after serum supplementation?

BenchStable media can be used for up to 2-4 weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

How long are BenchStable media good for after the bottle has been opened?

To reduce the chances of contamination and the impact of pH drift, we recommend using BenchStable media within 2-4 weeks after the bottle has been opened.

How should I store complete or serum-supplemented BenchStable media?

Complete BenchStable media (for example, media supplemented with 10% FBS) should be stored at 4 degrees C.

What is the shelf-life of BenchStable media?

BenchStable media have a 12 month shelf life, consistent with our standard basal media offerings.

For Research Use Only. Not for use in diagnostic procedures.