The Bovine Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit is designed for the quantitative detection of B-OHB in bovine serum and plasma samples using a competitive ELISA method This kit detects B-OHB with a sensitivity of 9 5 nmol/mL and provides a broad detection range from 50 nmol/mL to 800 nmol/mL making it suitable for monitoring ketosis and metabolic disorders in cattle The assay requires a sample volume of 50-100 L and delivers results within 1 to 5 hours Measurements are performed at an absorbance wavelength of 450 nm ensuring reliable and accurate quantification Ideal for veterinary research and clinical diagnostics this kit offers a standardized protocol for efficient analysis of B-OHB levels supporting metabolic health assessments in bovine populations