AdipoGen beta-Tubulin mAb AXO45

Supplier:  AdipoGen AG20B0085C100

Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications ICC, IHC, IP and WB. Clone AXO45. Isotype Mouse IgG1. The β-tubulin monoclonal antibody AXO45 was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin A.M. Callen, et al. 1994. The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.

Catalog No. 50-256-3369