Thermo Scientific Chemicals BICINE, 0.5M buffer soln., pH 8.5

Catalog No.	Quantity
AAJ61632AE	100 mL
AAJ61632AK	250 mL

Catalog No. AAJ61632AE Supplier Thermo Scientific Chemicals Supplier No. J61632AE

Description

CAS: 150-25-4 | C6H12NNaO4 | 185.16 g/mol

A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Solubility
Soluble in water (1M) at 20°C, ethanol (slightly), ether (slightly), and chloroform (slightly).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.

Specifications

Chemical Identifiers

• CAS: 150-25-4
• Molecular Formula: C6H12NNaO4
• Molecular Weight (g/mol): 185.16
• MDL Number: MFCD00004295
• InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M
• PubChem CID: 8761
• ChEBI: CHEBI:40957
• IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid
• SMILES: [Na+].OCCN(CCO)CC([O-])=O

• Concentration: 0.5 M
• pH: 8.5
• Physical Form: Liquid
• Quantity: 100 mL
• Solubility Information: Soluble in water (1M) at 20°C,ethanol (slightly),ether (slightly),and chloroform (slightly).
• Chemical Name or Material: BICINE buffer soln.

Safety and Handling

TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only