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Applied Biosystems™ BDT/POP/Buffer Bundle
Description
The BDT/POP/Buffer Bundle includes a BigDye Terminator 3.1 Cycle Sequencing Kit, POP-7 Polymer, Anode Buffer Containers, and Cathode Buffer Containers for your 3500 or SeqStudio Flex genetic analyzer. All products are offered in a single, easy-to-order bundle to provide key reagents for Sanger sequencing workflows.
BigDye Terminator v3.1 Cycle Sequencing Kit
The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions. The kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
POP-7 Polymer
POP-7 Polymer is a separation matrix for the 3500 and SeqStudio Flex DNA analyzers. It is customized for DNA sequencing and fragment analysis.
- Easy to use—pre-formulated and in a single-use pouch, which not only saves time but helps ensure reliability and consistency
- Includes radio frequency identification (RFID) label—easy to track important information such as part⁄lot number, samples remaining, and expiry dates (displayed on software dashboard)
- Robust formulation—formulated for sequencing and fragment analysis applications
- Replenishable—allow capillaries to be used multiple times
Cathode Buffer Containers
The Cathode Buffer Container (CBC) contains 1X running buffer to support all electrophoresis applications on 3500 and SeqStudio Flex series genetic analyzers. The container has two separate compartments: the left compartment provides the cathode buffer for electrophoresis and the right provides for a capillary wash and spent polymer waste ejection functionality between injections. The CBC is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the CBC is heat sealed with a plastic film, which should be removed prior to direct installation on to the instrument. Each package includes four individual containers.
Anode Buffer Containers
The Anode Buffer Container (ABC) contains 1X running buffer to support all electrophoresis applications on the 3500 and SeqStudio Flex series genetic analyzers. It is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat sealed with a plastic film, which should be removed prior to direct installation on the instrument. Each package includes four individual containers.
Specifications
Specifications
| Content And Storage | • BigDye Terminator v3.1 Ready Reaction Mix, 1 x 800 μL (Store at -15°C to -25°C) • M13 (-21) Primer, 1 tube (-15°C to -25°C) • pGEM Control DNA, 1 tube (-15°C to -25°C) • 5X Sequencing Buffer, 1 x 12 mL (-15°C to -25°C, or 2°C to 8°C) • POP-7 Polymer, 96 reactions (2°C to 8°C) • Cathode buffer container, 4 packs (2°C to 8°C) • Anode buffer container, 4 packs (2°C to 8°C) |
| Control Type | Plasmid DNA |
| Detection Method | Fluorescent Dye |
| Format | Kit |
| Isolation Technology | Enzymatic, Magnetic Bead |
| Sample Type | Purified DNA |
| Sequencing Type | Genome and DNA Sequencing |
| Enzyme | Proprietary |
| For Use With (Equipment) | 3500xL Genetic Analyzer, 3500 Genetic Analyzer |
| For Use With (Application) | Standard Sequencing |
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