Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit

Description

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications
• Sequence challenging templates and sequences more successfully
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Tackle Tough Sequences

With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy

With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow

BigDye™ Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

Specifications

• Content And Storage: • 200 μL Tube of BigDye™ Terminator v1.1 Ready Reaction Mix
  • Tube M13 (-21) Primer
  • Tube pREF-BDT™ Control DNA (200 ng/μL)
  • 1 mL Tube of 5x Sequencing Buffer (may be stored at 4°C)
  
  Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
• For Use With (Equipment): SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
• For Use With (Application): AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), Short PCR Product Sequencing, GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, De Novo Sequencing - Low to Mid Throughput
• Product Line: BigDye™ Terminator
• Product Type: Sequencing Buffer
• Quantity: 24 Reactions
• Technique: Fluorescent Dye Terminator Sequencing
• Template Compatibility: BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA

For Research Use Only. Not for use in diagnostic procedures.