Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

Description

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy

With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

Specifications

• Content And Storage: • 200 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix
  • 1 tube M13 (-21) Primer
  • 1 tube pREF-BDT™ Control DNA
  • 1 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
  
  Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
• Format: 12-well Strips, 8-well Strip, 96- or 384-well Plate
• For Use With (Equipment): SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
• For Use With (Application): AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
• Product Line: BigDye™ Terminator
• Product Type: Sequencing Buffer
• Quantity: 24 Reactions
• Technique: Fluorescent Dye Terminator Sequencing
• Template Compatibility: BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons

For Research Use Only. Not for use in diagnostic procedures.