Invitrogen™ Mouse BIGH3 (TGFBI) ELISA Kit

Catalog No.	Quantity
EMTGFBIX5	5 x 96 Tests
EMTGFBI	96 Tests

Catalog No. EMTGFBIX5 Supplier Invitrogen™ Supplier No. EMTGFBIX5

Description

ELISA

The Mouse BIGH3 (TGFBI) ELISA quantitates Ms BIGH3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BIGH3. Principle of the method The Mouse BIGH3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.

Specifications

• Accession Number: P82198
• Assay Range: 3-1000 ng/mL
• Assay Sensitivity: 3 pg/mL
• Calibration Range: 3 pg/mL to 1000 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects mouse beta-IGH3. Other species not determined.
• Sufficient For: 5 x 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 68kDa; AI181842; AI747162; beta ig-h3; Beta-ig; beta-ig-h3; betaig-h3; BIGH3; Big-h3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; kerato-epithelin; LCD1; MP70; MP78; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; transforming growth factor beta induced; transforming growth factor beta-induced 68kDa; transforming growth factor induced protein; transforming growth factor, beta induced; transforming growth factor, beta induced, 68 kDa; transforming growth factor, beta-induced, 68kD; transforming growth factor, beta-induced, 68kDa; Transforming growth factor-beta-induced protein ig-h368kDa; AI181842; AI747162; beta ig-h3; Beta-ig; beta-ig-h3; betaig-h3; BIGH3; Big-h3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; kerato-epithelin; LCD1; MP70; MP78; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; transforming growth factor beta induced; transforming growth factor beta-induced 68kDa; transforming growth factor induced protein; transforming growth factor, beta induced; transforming growth factor, beta induced, 68 kDa; transforming growth factor, beta-induced, 68kD; transforming growth factor, beta-induced, 68kDa; Transforming growth factor-beta-induced protein ig-h3; 68kDa; AI181842; AI747162; Beta-ig; big-h3
• Gene ID (Entrez): 21810
• Gene Symbol: TGFBI
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Beta ig-h3
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Angiogenesis
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: 68 kDa, AI181842, AI747162, Beta-ig, big-h3
• Target: TGFBI
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min