Bile Esculin Agar is used for the selective isolation and differentiation of group D streptococci from foods and is not intended for use in the diagnosis of disease or other conditions in humans.Bile Esculin Agar is based on the formulation described by Swan and further evaluated by Facklam and Moody. Rochaix first noted the value of esculin hydrolysis in the identification of enterococcci. Meyer and Schonfeld added bile to the esculin medium and demonstrated 61 of 62 enterococci strains were able to grow and hydrolyze esculin, while other streptococci could not.Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described as group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/bile-esculin-agar